β-II conformation of all-β proteins can be distinguished from unordered form by circular dichroism

The CD spectrum of certain all-β globular proteins resembles that of unfolded proteins with a characteristic negative band around 200 nm. The conformation of this class is tentatively termed β-II, which had two features that were absent for unfolded proteins. First, β-II proteins usually had CD bands due to aromatic side groups in the near-ultraviolet region. Second, the CD intensities both in the far- and in the near-uv region of these compact and rigid proteins usually showed a sharp transition upon thermal denaturation, whereas those of an unordered form changed linearly with rising temperature.     

Hwabyung: The construction of a korean popular illness among korean elderly immigrant women in the United States

The cultural construction of Hwabyung, a Korean culture-bound syndrome, is explored among a sample of 20 elderly Korean immigrant women in the United States. Hwabyung results when distressed emotions associated with the specifically Korean way of perceiving and reacting to intolerable and tragic life situations cause bodily symptoms by interfering with the harmony of Ki (vital energy). Korean elderly immigrants report a broad range of symptoms associated with Hwabyung; they less frequently report the epigastric mass, which had been considered the cardinal symptom by cosmopolitan and traditional medical writers. Hwabyung is treated holistically with psychosocial support from family, spiritual comfort, home and popular remedies, traditional Korean medicine, and biomedical treatments. Hwabyung provides a way of conceptualizing and resolving emotional distress through somatization among Korean elderly immigrant women.     

版纳鱼螈和双带鱼螈核型的比较研究

本文对版纳鱼螈的核型作了观察,并与双带鱼螈进行了比较。两者的核型基本一致,均为2n=42,但版纳鱼螈的NF=62,而双带鱼螈的NF=60;版纳鱼螈的染色体有中部着丝粒、亚中部着丝粒和端部着丝粒三种类型,而双带鱼螈则无亚中部着丝粒的染色体。     

急性缺氧性缺氧时心脏功能的改变

F_(IO_2)(吸入气氧浓度)为12.35、9.87及7.7l％,分别吸入10、8及5min时,心功能呈代偿性增强改变。F_(IO_2)为9.37％、吸入20min时心功能的变化趋势与9.87％8min时仍基本相同。继发性缺二氧化碳对缺氧引起的心功能代偿性增强,在一定程度上起抵消作用。F_(IO_2)为9.87％时的缺氧程度约相当于18km高空加压供氧总压值为15.3kPa(115mmHg)时的缺氧。单纯从缺氧因素考虑,将总压值由常用的17.3kPa(130mmHg)降低为15.3kPa是可允许的。     

粉纹夜蛾核型多角体病毒诱导的胸苷激酶特性研究

粉纹夜蛾(Trichoplusia ni)核型多角体病毒(TnNPV)感染草地贪夜蛾(Spodop-tera frugiperda)细胞后能诱导提高胸苷激酶的活性。不论是正常或感染细胞中的胸苷激酶都可将脱氧胞苷磷酸化,酶活最适pH及Mg~( )离子浓度值基本相同,DEAE-纤维素和Cibacron blue-sepharose柱层析所得酶活性图谱亦相似。TnNPV在胸苷激酶缺陷型的草地贪夜蛾细胞中能正常复制,但被感染细胞不具有胸苷激酶活性。由此可以确定,经TnNPV感染诱导后,活性有所提高的胸苷激酶不是病毒基因编码的。     

植物释放氧化亚氮的研究

氧化亚氮(N_2O)是大气的微量成分之一。多年的测定表明,它在大气中的浓度正以0.2％左右的年增长率在增加。N_2O具有“温室效应”并能催化大气同温层中臭氧保护层的破坏,从而可能带来全球性生态环境的重大变化而受到世人的极大关注。各国科     

Genetic mapping studies of 40 loci and 23 cosmids in chromosome 11p13-11g13, and exclusion of μ-calpain as the multiple endocrine neoplasia type 1 gene

Forty loci (16 polymorphic and 24 non-polymorphic) together with 23 cosmids isolated from a chromosome 11-specific library were used to construct a detailed genetic map of 11p13-11g13. The map was constructed by using a panel of 13 somatic cell hybrids that sub-divided this region into 19 intervals, a meiotic mapping panel of 33 multiple endocrine neoplasia type 1 (MEN1) families (134 affected and 269 unaffected members) and a mitotic mapping panel that was used to identify loss of heterozygosity in 38 MENI-associated tumours. The results defined the most likely order of the 16 loci as being: 11pter-D11S871(D11S288, D11S149)-11cen-CNTF-PGA-ROM1-D11S480-PYGM-SEA-D11S913-D115970-D11S97-D11S146-INT2-D11S971-D11S533-11gter. The meiotic mapping studies indicated that the most likely location of the MEN1 gene was in the interval flanked by PYGM and D11S97, and the results of mitotic mapping suggested a possible location of the MEN1 gene telomeric to SEA. Mapping studies of the gene encoding μ-calpain (CAPN1) located CAPN1 to llg13 and in the vicinity of the MEN1 locus. However, mutational analysis studies did not detect any germ-line CAPN1 DNA sequence abnormalities in 47 unrelated MEN1 patients and the results therefore exclude CAPN1 as the MEN1 gene. The detailed genetic map that has been constructed of the 11p13-11g13 region should facilitate the construction of a physical map and the identification of candidate genes for disease loci mapped to this region.     

山茶科紫茎属和舟柄茶属的系统学研究

本文对山茶科紫茎属和舟柄茶属进行了深入细致的系统学研究,藉助形态学、古植物学、孢粉学,细胞学和解剖学资料澄清了两属的分合问题,证实两属在各方面具有较大相似性,并且各分类特征存在广泛的联系而无法分开,从而赞同H．K．Airy Shaw,J．R．Sealy及S．A.Spongberg的主张,即将这两属合并。在此基础上本文提出了世界范围广义紫茎属下分类系统。属下新系统根据花柱合生程度、花序类型,苞片与萼片的形状以及两者的相对长度等特征,分为两个亚属,五个组,同时对该属种类进行修订。该属共有23种5变种,本文发表新组1个,新名称2个,新组合9个,新异名10个,新种1个,并附有分种检索表。广义的紫茎属为东亚-北美间断分布类型,中国南部和西南部是该属的起源中心和高度分化中心。根据化石资料推断,该属起源于早白垩纪,在第三纪以前于整个劳亚古陆上呈广泛而连续的分布,后因冰川及造山运动的影响,从而形成现在的分布格局。     

Tobacco (Nicotiana tobaccum) nuclear transgenics with high copy number can express NPTII driven by the chloroplast psbA promoter

A chloroplast expression vector containing the NPTII gene under the control of apsbA promoter (psbA-NPTII) was constructed, and was biolistically delivered into both suspension cells and leaf strips of tobacco (Nicotiana tabaccum). Analyses of subsequently recovered kanamycin-resistant transgenic plants indicate that the psbA-NPTII gene was not located in the chloroplast, but was in the nucleus in very high copy number. This conclusion was based upon results from: (1) Southern hybridization analyses of chloroplast and nuclear DNAs using NPTII, chloroplast-marker, and nuclear-marker probes; (2) pulse-field gel electrophoresis; and (3) kanamycin screening of sexual progenies. This study suggests that the nuclear expression of the NPTII gene may have been associated with many copies of the psbA-NPTII construction. Very high copy number in the nucleus might either allow NPTII expression from the otherwise inadequate psbA promoter, or might increase the chance of recombining with upstream tobacco regulatory sequences.     

嗜热链球菌培养条件的研究

将嗜热链球菌（Ｓｔｒｅｐｔｏｃｏｃｃｕｓｔｈｅｒｍｏｐｈｉｌｕｓ）在６种固体培养基及７种液体培养基中的生长情况进行了比较,结果表明,改良ＩＲＩＥ固体培养基上的溶钙圈大,活菌数最多;在７种液体培养基中亦以改良ＩＲＩＥ的ＯＤ值最高,活菌最多,达８×１０^８／ｍｌ以上。以此培养基做生长曲线,该菌的平衡期为１２～１６ｈ。